As expected, suppression of Egr 1 activity caused downregulation of Atg4B at the message and protein levels (Figure 4b). Thus, we cloned the putative human Atg4B promoter in a luciferase reporter. Luciferase assays revealed that Egr 1 was critical to Atg4B promoter activity through binding to region ( to under stimulation by IR exposure because the mutations in these binding sites exhibited lower promoter activity (Figure 4c).
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